Bromelain

about 1200 GDU / g, for biochemistry
Made from stems of young pineapple (Bromeliaceae).
Bromelin

Melting point (mp) approx. 60 ° C

Storage temp .: +4 ° C
WGK 3
CAS No. [9001-00-7]
EC no. 232-572-4

Highly active thiol proteinase (non-specific cystein protease), glycoprotein. Enzyme from Bromeliaceae, used in biochemistry and for meat tenderising. Preferable digestion at lysin, alanin, tyrosin and glycin. Degrades hemoglobin, gluten, collagen, globulin, casein and gelatin at pH 4-9. Cream-colored, hygroscopic powder. Bromelain is activated by cystein, bisufite salts, NaCN, Na2S, H2S and benzoate. The definition pH optimum depends on substrate and reaction parameters. pH stability of bromelain increases drastically after binding to the substrate.

One GDU (gelatin digestion unit) will hydrolysis 1.0 mg of amino nitrogen from gelatin in 20 minutes at pH 4.5 at 45 ° C. One bromelain tyrosine unit (BTU) corresponds to 1.1 GDU. 1200 GDU / g correspond to approx. 2.4 FIP units / mg.

Applications:

pH working range: 4.0-8.0

pH optimum: 5-6. Stabile at pH 3.0-6.5

Temperature optimum: 50-65 ° C

Inhibitors: Hg ++, Ag ++, Cu ++, antitrypsin, estatin, iodacetate, TLCK, TPCK, and temperatures of over 70 ° C

Stock solution: 10 mg / ml in H2O

Unsoluble in organic solvents (acetone, ethanol, methanol, etc.)

Working concentration: substrate dependent

$$$$$

Hazard statements
H315 Causes skin irritation.
H319 Causes serious eye irritation.
H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled
cause.
H335 May cause respiratory irritation.

Safety recommendations
Precautions - prevention
P280 Wear protective gloves / eye protection.

Precautions - response
P302 + P352 IF ON SKIN: Wash with plenty of soap and water.
P304 + P340 IF INHALED: Remove to fresh air and keep at rest in a position suitable
makes breathing easier.
P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for a long period of time
amount of minutes; remove contact lenses, if possible; keep rinsing.
P342 + P311 In case of respiratory symptoms: Call a POISON CENTER / doctor.