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Trypsin 5.000 USP-U/mg

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Technical specifications

Information

A pancreatic serine protease with a specificity for peptide bonds between basic amino acids arginine or lysine and another non-specific amino acid.

Trypsin is frequently used to suspend cells which grow as monolayers on surfaces. Longer exposure to trypsin can damage cells, so that an inhibitor (serum or trypsin inhibitor from soya beans) should be added upon completion.

Directions for use

pH optimum 7.2-8.5.

Unit definition

One USP Unit will cause an increase in absorption (253 nm) per minute of 0.003 at 25 °C and pH 7.6 using BAEE as substrate.
One (international) Unit (U, 255 nm, BAEE, pH 8) is approx. 90 USP U.
One TAME Unit is approx. 20 USP Units.
One NF Unit is one USP Unit.
Ten „BAEE Units“ are approx. 3.3 USP Units.

Molar mass (M) ~24,000 g/mol
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1
CAS No. [9002-07-7]
EG-Nr. 232-650-8

Serine protease isolated from porcine pancreas, lyophilised.

A pancreatic serine protease with a specificity for peptide bonds between basic amino acids arginine or lysine and another non-specific amino acid.

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Hazard statements

H315 Causes skin irritation.
H319 Causes serious eye irritation.
H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled.
H335 May cause respiratory irritation.

Precautions - prevention

P280 Wear protective gloves / protective clothing / eye protection / face protection.

Precautions - response

P302 + P352 IF ON SKIN: Wash with plenty of soap and water.
P304 + P341 IF INHALED: In case of breathing difficulties, remove victim to fresh air and keep at rest in a position comfortable for breathing.
P305 + P351 + P338 IF IN EYES: Rinse cautiously with water for several minutes. remove contact lenses, if possible; keep rinsing.
P342 + P311 In case of respiratory symptoms: Call a POISON CENTER / doctor.