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iScope fluorescence

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For biological and medical applications fluorescence microscopes are used to observe certain parts of living cells and tissues with help of fluorophores. One or more fluophores are added to the parts of the specimen to be observed. When the fluorophores are exposed to so-called exicitation light, the excitation energy is absorbed by the fluorophore that start emitting so-called emission light that can be observed by the microscope. One can distinguish tumor cells from other cells, follow biological processes or prove the presence or absence of antibodies, etc

The excellent optics and mechanical components make the iScope a state-of-the art microscope that is suitable for fluorescence applications in Life Science and Biotechnical fields

The iScope fluorescence microscopy can be supplied with:

• 6-position rotating attachment with 100 W mercury vapor light source
• 3-position slider attachment with 100 W mercury vapor light source
• 1-position slider attachment with a 3 W NeoLED 465-475 nm blue light source
• 1-position slider attachment with a 3 W NeoLED 520-530 nm green light source

Icons are used to indicate the several functions of a microscope.
Click and find more details about the meaning of each icon

Highlights

HWF 10x/22 mm eyepieces
Binocular and trinocular models
Optional ergonomic tilting head
Quintuple revolving nosepiece
E-plan, plan and plan Fluarex IOS corrected objectives
187/230 x 140 mm rackless stage with integrated X-Y stage
Optional ceramic stage
Diascopic intensity adjustable 3 W NeoLED Köhler illumination
Epi-fluorescence 100 W HBo mercury vapor
Blue and Green LED fluorescence
iCare sensor for energy saving
CSS - Cable Storage System

6-positions rotating attachment

With 100 W mercury vapor light source
The 6-positions rotating fluorescence attachments are supplied with a standard 100 W HBO mercury vapor light source for broad powerful light spectrum. Supplied with standard fluorescence filters sets for excitation in the blue and green light spectrum and a UV protection shield

MODELS	Binocular	Trinocular	WF 10x/22 mm	Diopter adjustable on left tube	Diopter adjustable on both tubes	E-plan EPLi 4x/10x/S40x and S100x objectives	Plan PLi 4x/10x/S40x and S100x objectives	Plan PLFi 4x/10x/S40 and S100x objectives	100 W HBO light source	Filter sets (blue&green)	NeoLED illumination
IS.3152‑EPLi/6	•		•	•		•			•	•	•
IS.3153‑EPLi/6		•	•	•		•			•	•	•
IS.3152‑PLi/6	•		•		•		•		•	•	•
IS.3153‑PLi/6		•	•		•		•		•	•	•
IS.3152‑PLFi/6	•		•		•			•	•	•	•
IS.3153‑PLFi/6		•	•		•			•	•	•	•

3-positions slider attachment

With 100 W mercury vapor light source
The 3-positions slider fluorescence attachments are supplied with standard 100 W HBO mercury vapor light source for broad powerful light spectrum. Supplied with standard fluorescence filters sets for blue and green excitation and a UV protection shield

MODELS	Binocular	Trinocular	WF 10x/22 mm	Diopter adjustable on left tube	Diopter adjustable on both tubes	E-plan EPLi 4x/10x/S40x and S100x objectives	Plan PLi 4x/10x/S40x and S100x objectives	Plan PLFi 4x/10x/S40x and S100x objectives	100 W HBO light source	Filter sets (blue&green)	NeoLED illumination
IS.3152‑EPLi/3	•		•	•		•			•	•	•
IS.3153‑EPLi/3		•	•	•		•			•	•	•
IS.3152‑PLi/3	•		•		•		•		•	•	•
IS.3153‑PLi/3		•	•		•		•		•	•	•
IS.3152‑PLFi/3	•		•		•			•	•	•	•
IS.3153‑PLFi/3		•	•		•			•	•	•	•

1-position slider attachment

Blue: single position slider fluorescence attachment with a 3 W NeoLED™ light source for blue excitation (/LB)
Green: single position slider fluorescence attachment with a 3 W NeoLED™ light source green excitation (/LG)

MODELS	Binocular	Trinocular	WF 10x/22 mm	Diopter adjustable on left tube	Diopter adjustable on both tubes	E-plan EPLi 4x/10x/S40x and S100x objectives	Plan PLi 4x/10x/S40x and 100x objectives	Plan PLFi 4x/10x/S40x and S100x objectives	Blue excitation 460-495 nm	Green excitation 520-530 nm	NeoLED illumination
IS.3152‑EPLi/LB	•		•	•		•			•		•
IS.3153‑EPLi/LB		•	•	•		•			•		•
IS.3152‑PLi/LB	•		•		•		•		•		•
IS.3153‑PLi/LB		•	•		•		•		•		•
IS.3152‑PLFi/LB	•		•		•			•	•		•
IS.3153‑PLFi/LB		•	•		•			•	•		•
IS.3152‑EPLi/LG	•		•	•		•				•	•
IS.3153‑EPLi/LG		•	•	•		•				•	•
IS.3152‑PLi/LG	•		•		•		•			•	•
IS.3153‑PLi/LG		•	•		•		•			•	•
IS.3152‑PLFi/LG	•		•		•			•		•	•
IS.3153‑PLFi/LG		•	•		•			•		•	•

EYEPIECES
Extended EWF 10x/22 mm eyepieces, Ø 30 mm tubes. Eyepieces can be secured to the tubes. The trinocular head of the Plan infinity corrected models (PLi and PLFi) has a light path selector (100:0 / 50:50) and ± 5 diopter adjustments on both tubes.

The trinocular head of the E-Plan infinity corrected models (EPLi) has a fixed built-in light path beamsplitter (50:50) and ± 5 diopter adjustment on the left tube

HEAD
• Binocular and trinocular Siedentopf type heads with 30° inclined tubes. Interpupillary distance from 48 to 76 mm
• A unique rotating system allows the ergonomical positioning of both tubes in a high (431 mm) and in a low position (397 mm)
• The trinocular head has a 23.2 mm photo tube. This fits all C-mount adapters supplied with Euromex cameras

To order an iScope® with tilting head use reference IS.3158-xxx (Epli/Pli/PLFi)
To order an iScope® with tilting trino head use reference IS.3159-xxx (EPLi/Pli/PLFi)

NOSEPIECE
Revolving quintuple reversed nosepiece on ball bearings

OBJECTIVES
The EPLi and PLi objectives are suitable for Blue and Green standard filter sets and other filter sets in the visible spectrum.
The plan Fluarex PLFi ™ objectives are suitable for the entire spectrum including Ultra Violet and Violet

E-Plan EPLi IOS	4x/0.10	10x/0.25		S40x/0.65		S100x/1.25 oil
Plan PLi IOS	4x/0.10	10x/0.25	20x/0.40*	S40x/0.65	S60x/0.85*	S100x/1.25 oil
Plan PLFi IOS	4x/0.15	10x/0.35	20x/0.60*	S40x/0.75		S100x/1.30 oil

^* optional
All optics are anti-fungus treated and anti-reflection coated for maximum light throughput

STAGE
187/230 x 140 mm rackless stage equipped with double slide holder and integrated 79 x 52 mm mechanical stage. The iScope® rackless stage has no protruding parts, enabling more smooth movements and is safer

Optional: 220 x 157 mm ceramic stage with integrated 75 x 50 mm mechanical stage protects with a very tough ceramic surface, against marks and scratches. The surface also ensures stray light not to be reflected from the stage into the objective

CONDENSER FOR BRIGHTFIELD
The standard height adjustable Abbe N.A. 1.25 condenser for brightfield with magnification indication.
Also accepts sliders with phase annuli suitable for plan phase 10x/20x or S40x/ S100x oil immersion IOS objectives for economical phase contrast. A slider with a simple dark stop for darkfield up to 400x magnification is also available for this condensor

CONDENSER FOR ZERNIKE PHASE CONTRAST
An optional height adjustable Zernike NA. 1.25 phase contrast disc condenser with darkfield stop position for darkfield contrast observation up to 400x magnification and plan phase 10/20/S40x and S100x oil immersion PLPHi IOS objectives

FOCUSING
Coaxial coarse and fine adjustments, 200 graduations, 1 μm per graduation, 200 μm per rotation, total travel range is approximately 24 mm. Supplied with an adjustable rack stop to prevent damage to sample and objectives. The coarse adjustments are equipped with friction control

ILLUMINATION
3 W adjustable Köhler NeoLED diascopic illumination with internal 100-240 V power supply

The innovative NeoLED design offers larger apertures, allowing the optical system of the iScope microscope to produce images at higher resolutions, very close to the theoretical diffraction limit of the optics. Other benefits of the NeoLED is the low energy consumption, no heating and a long operating lifetime

The Köhler illumination on models with IOS plan and plan phase infinity corrected system provides high contrast and a maximum achievable resolving power of the optics. Also available for other models as an option

ILLUMINATION FOR FLUORESCENCE

The 6-position rotating and 3-position slider fluorescence attachments are supplied with:
• a 100 W HBO mercury vapor light source for broad powerful light spectrum. Including an 100-240 V external power supply with indication of the life span of the HBO bulb

• Standard fluorescence filters sets for
- blue excitation (EX460- 490 (BP) DM520 EM500 (LP)
- green excitation (EX510-550 (BP) DM580 EM570 (LP) and a UV protection shield

The iScope® fluorescence microscopes equipped with a 100 W mercury vapor light source can be supplied with more specific, optimized and application related fluorescence filters sets (see next pages).

Two single position LED fluorescence attachments:
- Blue: EX 460-495 DM 505 EM 510-560 LP filter set and a 3 W 465-475 nm LED for blue excitation
- Green: EX 510-550 DM570 EM 590 LP filter set and a 3 W 520-530 nm LED light source for green excitation

Both LED versions are supplied with a 100-240 Vac/7.5 Vdc external power supply

STANDARD GENERAL PURPOSE FLUORESCENCE FILTER SETS

IS.9745

IS.9745 - Blue excitation filter set
EX: 460-490 nm bandpass DM: 520 nm EM: 500 nm longpass

IS.9746

IS.9746 - Green excitation filter set
EX: 510-550 nm bandpass DM: 580 nm EM: 570 nm longpass

IS.9747

IS.9747 - UV excitation filter set
EX: 330-385 nm bandpass DM: 420 nm EM: 400 nm longpass

IS.9748

IS.9748 - Violet excitation filter set
EX 400-410 nm bandpass DM 455 nm EM 455 nm longpass

ICARE SENSOR
The unique iCare Sensor is developed to avoid unnecessary loss of energy. The illumination of the microscope automatically switches off shortly after microscopists step away from their position

CSS - CABLE STORAGE SYSTEM
iScope allows users to easily insert the power cable into the back of the instrument, which enables easy storage. The integrated carrying grip at the back of the microscope ensures safe transportation of the microscope

CONFIGURATIONS
The iScope microscope is available in many configurations and can be upgraded with a variety of contrast types

PACKAGE CONTENT
Supplied with power cord, dust cover, a spare fuse, user manual and 5 ml immersion oil for models with S100x objective. Models with 100 W mercury vapor light source have a UV protection shield. All packed in polystyrene boxes

	PB.5276 Microscoop-onderhoudsset, 16 onderdelen: schoonmaakpenseel, 6-delige schroevendraaiersset, blaasbalgje, 3-delige inbusset (1,5, 2, 2,5 mm), lensreinigingsvloeistof 20 ml, reinigingsdoek 140 x 140 mm, 100 stuks lenstissues, tube onderhoudsvet, 10 ml fles olie, verpakt in een overzichtelijke opbergdoos
	PB.5157-B Objectglaasjes 76 x 26 mm, wit glas, kanten geslepen. Een kant blauw. Per 50 stuks verpakt
	PB.5157-W Objectglaasjes 76 x 26 mm, wit glas, kanten geslepen. Per 50 stuks verpakt
	IS.5700 Ergonomische kantelbare kop 5 tot 35°. Voor iScope oneindig gecorrigeerde optische systemen
	IS.6010 EWF 10x/20 mm oculair, Ø 23,2 mm tubus
	IS.6010-C EWF 10x/20 mm oculair met kruisdraad, Ø 23,2 mm tubus
	IS.6010-P EWF 10x/20 mm oculair met aanwijsnaald, Ø 23.2 mm tubus
	IS.6010-CM EWF 10x/20 mm oculair met 10/100 micrometer en kruisdraad, Ø 23,2 mm tubus
	IS.6015 WF 15x/16 mm oculair, Ø 23,2 mm tubus
	IS.6020 WF 20x/12 mm oculair, Ø 23,2 mm tubus
	IS.6099 Paar oogschelpen, Ø 23,2 mm tubus
	IS.6210 EWF 10x/22 mm oculair, Ø 30 mm tubus
	IS.6210-C EWF 10x/22 mm oculair met 10/100 micrometer en kruisdraad, Ø 30 mm tubus
	IS.6210-P EWF 10x/22 mm oculair met 10/100 micrometer en kruisdraad, Ø 30 mm tubus
	IS.6210-CM EWF 10x/22 mm oculair met 10/100 micrometer en kruisdraad, Ø 30 mm tubus
	IS.6212 WF 12,5x/15 mm oculair, Ø 30 mm tubus
	IS.6215 WF 15x/16 mm oculair, Ø 30 mm tubus
	IS.6220 WF 20x/12 mm oculair, Ø 30 mm tubus
	IS.6299 Paar oogschelpen, Ø 30 mm tubus
	IS.7104 E-plan EPL 4x/0,10 objectief
	IS.7110 E-plan EPL 10x/0,25 objectief
	IS.7120 E-plan EPL 20x/0,40 objectief
	IS.7140 E-plan EPL S40x/0,65 objectief
	IS.7160 E-plan EPL S60x/0,85 objectief
	IS.7100 E-plan EPL S100x/1,25 objectief
	IS.7710 Plan PLPH 10x/0,25 fasecontrast objectief
	IS.7720 Plan PLPH 20x/0,40 fasecontrast objectief
	IS.7740 Plan PLPH S40x/0,65 fasecontrast objectief
	IS.7700 Plan PLPH S100x/1,25 fasecontrast objectief
	IS.8804 E-plan EPLi 4x/0,10 IOS oneindig gecorrigeerd objectief
	IS.8810 E-plan EPLi 10x/0,25 IOS oneindig gecorrigeerd objectief
	IS.8840 E-plan EPLi S40x/0,65 IOS oneindig gecorrigeerd objectief
	IS.8800 E-plan EPLi S100x/1,25 IOS oneindig gecorrigeerd objectief
	IS.7202 Plan PLi 2x/0.05 IOS oneindig gecorrigeerd objectief, geen dekglascorrectie. Werkafstand 18,3mm
	IS.7204 Plan PLi 4x/0,10 IOS oneindig gecorrigeerd objectief
	IS.7210 Plan PLi 10x/0,25 IOS oneindig gecorrigeerd objectief
	IS.7220 Plan PLi 20x/0,40 IOS oneindig gecorrigeerd objectief
	IS.7240 Plan PLi S40x/0,65 IOS oneindig gecorrigeerd objectief
	IS.7260 Plan PLi S60x/0,85 IOS oneindig gecorrigeerd objectief
	IS.7200 Plan PLi S100x/1,25 IOS oneindig gecorrigeerd objectief
	IS.7200-I Plan PLi S100x/1,25 IOS oneindig gecorrigeerd objectief met geïntergreerde irisdiafragma
	IS.8910 Plan PLPHi 10x/0,25 fasecontrast IOS oneindig gecorrigeerd objectief
	IS.8920 Plan PLPHi 20x/0,40 fasecontrast IOS oneindig gecorrigeerd objectief
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