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Inhibitor Cocktail His-tag
Information
Density (D) 1 g/cm³
Boiling point (bp) >180 °C
Flash point (flp) >80 °C
Storage temp. -20 °C
Transport temp. cooled
WGK 1
Boiling point (bp) >180 °C
Flash point (flp) >80 °C
Storage temp. -20 °C
Transport temp. cooled
WGK 1
Broad range cocktail of protease inhibitors for extraction of His-tagged proteins. Without EDTA.
Storage of inhibitor solution: -20 °C max. 6 months.
Storage of inhibitor solution: -20 °C max. 6 months.
Inhibitor cocktail for inhibition of a broad range of proteases. Inhibits serine proteases, aminopeptidase B, leucine-aminopeptidases, cysteine proteases, aspartic proteases, and metalloendopeptidases.
Recommended as cocktail for protease inhibition particularly for extraction of His-tagged proteins from bacterial extracts. EDTA-free to allow optimal Ni++- or Co++-his binding in affinity chromatography.
Gebruiksinstructies
Recommended dilution approx. 1:100.
Provided as stock-solution in DMSO.
Concentrations per vial are:
AEBSF: 100 mM
Bestatin: 5 mM
-64: 1.5 mM
Pepstatin A: 0.2 mM
Phosphoamidon: 2 mM
